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Apologies if I missed this, but can Automations be defined in a configuration file (so it can be under version control) or only within the Prefect UI?

you can write them as JSON and then modify them through the API, I'll get you a link

api docs for creating an automation <https://app.prefect.cloud/api/docs#tag/Automations/operation/create_automation_api_accounts__account_id__workspaces__workspace_id__automations__post>

and docs on the syntax and rules for writing automation trigger json
<https://docs.prefect.io/latest/concepts/automations/#custom-triggers>