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Is it possible to assign work queues to deployment runs when starting the flow run, vs when creating the deployment?

yes you can pass a work queue name when invoking a deployment:

```    from prefect.deployments import run_deployment
    
    run_deployment(
        ...
        work_queue_name="..."
    )```

Sweet! Would that override what the deployment was created with? I suppose I can test that out...

It won't override the entire deployment, it will just use that work queue for that single run