The Prefect Helm Chart can be used to deploy Prefect workers, as described in this
guide. However, the documents do not explicitly mention deploying different workers using the same release or deploying workers for different work-pools using a single release of the Prefect-worker Helm chart.
Work pools and workers bridge the Prefect orchestration environment with your execution environment. When a deployment creates a flow run, it is submitted to a specific work pool for scheduling. A worker running in the execution environment can poll its respective work pool for new runs to execute, or the work pool can submit flow runs to serverless infrastructure directly, depending on your configuration. You can read more about this
here.
For more specific information, you may want to check the Prefect community or the official Prefect documentation.